Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.
نویسندگان
چکیده
PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.
منابع مشابه
PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.
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ورودعنوان ژورنال:
- Journal of microbiological methods
دوره 80 3 شماره
صفحات -
تاریخ انتشار 2010